Hard tissue repair and regeneration trials have been carried out using several approaches and strategies. However, evidence of utilizing the immunological approach is scarce.
It is important to understand the precise mechanism regulating hard tissue formation through identifying novel surface molecules expressed by the hard tissue-forming cells like the bone forming- osteoblasts and the dentin-forming odontoblasts.
The lecture will focus on using B-cell hybridoma technology as one of the techniques that could be utilized for the production of highly specific monoclonal antibodies detecting novel cell-surface molecules contributing to the fine regulation of hard tissue formation. The lecture will delve into one of the Immunological surveys that lead to the generation of a monoclonal antibody A7 (A7 MAb) highly specific to a unique cell-surface molecule in the cells of the osteoblast-lineage. Moreover, this antibody detected the same membrane protein in odontoblasts. This novel A7-MAb could be a promising immunological tool for enrichment and purification of functional odontoblasts directly from dental pulp tissues in regenerative dentistry.